Yield-stress gel supports printing of 3D biological tissues

By placing tiny aggregates of cells inside yield-stress gels, researchers have shown they can print biological tissue in complex 3D shapes

Original source: Materials Today

The eventual creation of replacement biological parts requires fully 3D capabilities that 2D and 3D thin-film bioprinting cannot currently supply. Now, using a yield stress gel, engineers at Penn State have shown they can place tiny aggregates of cells exactly where they want to, allowing them build the complex shapes that will be necessary for replacing bone, cartilage and other tissues.

“The reason why this is important is that the current cell aggregate bioprinting techniques can’t make complicated configurations, and is mostly in 2D and 3D thin films or simple configurations,” said Ibrahim Ozbolat, associate professor of engineering science and mechanics. “If we want complicated 3D, we need a supportive field.”

To provide that supportive field, the engineers chose a yield-stress gel, as they report in a paper in Communication Physics. Yield stress gels are unusual in that without stress they are solid gels, but under stress, they become liquid.

The researchers employed an aspiration-assisted bioprinting system that they demonstrated earlier this year to pick up aggregates of cells and place them precisely inside the gel. The stress of the aspiration nozzle against the gel liquefies it, but once the aspiration nozzle releases the cell aggregates and withdraws, the gel returns to solid again, self-healing. The tiny balls of cells rest upon each other and self-assemble, creating a solid tissue sample within the gel.

The researchers can place different types of cells together, in small aggregates, to form the required shape with the required function. Geometric shapes, like the cartilage rings that support the trachea, could also be suspended within the gel.

“We tried two different types of gels, but the first one was a little tricky to remove,” said Ozbolat. “We had to do it through washing. For the second gel, we used an enzyme that liquefied the gel and removed it easily.”

“What we are doing is very important because we are trying to recreate nature,” said Dishary Banerjee, a postdoctoral researcher in engineering science and mechanics. “In this technology, it is very important to be able to make free-form, complex shapes from spheroids.”

The researchers used a variety of approaches, creating theoretical models to get a physical understanding of what was happening. They then used experiments to test if this method could produce complex shapes.